Journal: Frontiers in Pharmacology

Article Title: Integrated network pharmacology and experimental verification to reveal the mechanisms of curcumin in the treatment of colorectal cancer

doi: 10.3389/fphar.2025.1703562

Figure Lengend Snippet: Venn diagram of curcumin targets, CRC-related genes, and DEGs from curcumin-treated DLD1 cells.

Article Snippet: The human CRC cell line DLD1 (ATCC, United States) was cultured as described and seeded in 96-well plates to reach 80% confluence before treatment.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Integrated network pharmacology and experimental verification to reveal the mechanisms of curcumin in the treatment of colorectal cancer

doi: 10.3389/fphar.2025.1703562

Figure Lengend Snippet: Curcumin promotes ferroptosis and regulates Wnt/β-catenin signaling pathway in vitro . (A) Representative images of transmission electron microscopy observation of mitochondria; curcumin induced reduced mitochondrial volume and increased membrane density in HCT116 cell lines (scale bar: 2 μm and 500 nm); (B) Relative mitochondrial area in HCT116 cell lines. (C) Relative mitochondrial density in HCT116 cell lines. (D–E) Curcumin induced the accumulation of Lipid ROS in CRC cells. Dose-dependent accumulation of Lipid ROS in HCT116 and DLD1 cells treated with curcumin (5–20 μm). (F–G) Curcumin effectively inhibited the expression of SLC7A11 and GPX4, downregulated level of β-catenin and promoted phosphorylation of GSK3β at Ser9. *P < 0.05, **P < 0.01 and ***P < 0.001.

Article Snippet: The human CRC cell line DLD1 (ATCC, United States) was cultured as described and seeded in 96-well plates to reach 80% confluence before treatment.

Techniques: In Vitro, Transmission Assay, Electron Microscopy, Membrane, Expressing, Phospho-proteomics

Journal: iScience

Article Title: SLC7A11-specific CAR-T cell therapy potently targets colorectal and pancreatic cancer

doi: 10.1016/j.isci.2025.113713

Figure Lengend Snippet: Antibody design and affinity evaluation (A) The procedures for generating murine monoclonal antibody using hybridoma technology. (B) The qPCR experiment confirmed the successful knockdown of SLC7A11 expression levels in DLD1 cells mediated by lentivirus ( n = 3). (C) The specificity of the antibody targeting SLC7A11 was validated using flow cytometry ( n = 4). (D) The affinity between 1A4 and SLC7A11 was confirmed through SPR analysis. (E) The affinity between humanized 1A4 and SLC7A11 was confirmed through SPR analysis. The SLC7A11 expression level data by qPCR were analyzed by t test. The MFI of SLC7A11 data was analyzed by ANOVA test. Data were presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The human colorectal cancer and pancreatic cell lines DLD1 (RRID: CVCL_0248), HT29 (RRID: CVCL_0320), HCT116 (RRID: CVCL_0291), PANC-1 (RRID: CVCL_0480) and MIA PaCa-2 (RRID: CVCL_0428) were obtained from the American Type Culture Collection.

Techniques: Knockdown, Expressing, Flow Cytometry

Journal: iScience

Article Title: SLC7A11-specific CAR-T cell therapy potently targets colorectal and pancreatic cancer

doi: 10.1016/j.isci.2025.113713

Figure Lengend Snippet: The cytotoxicity assays in vitro by CCK8, LDH, and MFI detection (A) The survival rate of target cells after 24 h of co-culture ( n = 5). (B) The lysis rates of target cells after 24 h of co-culture with UTD, MOCK, and CAR-T cells ( n = 3). (C) The co-culture figures under the fluorescence microscope for the Mock group and CART group ( n = 3). Scale bars represent 50 μm. (D andE) The cytotoxic effects of DLD1 cells by measuring the fluorescence intensity after co-culturing ( n = 3). Scale bars represent 50 μm. Data were analyzed by ANOVA test. The CCK8 data and MFI data were presented as mean ± SD. The LDH data were presented as mean ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The human colorectal cancer and pancreatic cell lines DLD1 (RRID: CVCL_0248), HT29 (RRID: CVCL_0320), HCT116 (RRID: CVCL_0291), PANC-1 (RRID: CVCL_0480) and MIA PaCa-2 (RRID: CVCL_0428) were obtained from the American Type Culture Collection.

Techniques: In Vitro, Co-Culture Assay, Lysis, Fluorescence, Microscopy

Journal: Biomedicines

Article Title: Daily Profile of miRNAs in the Rat Colon and In Silico Analysis of Their Possible Relationship to Colorectal Cancer

doi: 10.3390/biomedicines13081865

Figure Lengend Snippet: Daily pattern in expression of miR-150-5p and its target genes in DLD1 cells: ( A ) expression of miR-150-5p, bcl2 , and myb during 24 h cycle in DLD1 cells synchronized by serum shock; ( B ) efficiency of transfection by miR-150-5p mimic or mimic administered together with inhibitor in DLD1 cells. oligo—cells transfected with miR-150-5p inhibitor and/or mimic, nc—cells transfected with control sequence(s). Cells transfected with experimental oligos were compared to nc, * p < 0.05—unpaired t -test. Effect of miR-150-5p, miR-150-5p inhibitor, and control oligos on bcl2 ( C ) and myb ( D ) expression in DLD1 cells. 150—miR-150-5p mimic, 150 + i—miR-150-5p mimic administered together with inhibitor; nc m—control sequence for mimic, nc m + i—control sequences for mimic and inhibitor administered together. Expression in cells transfected with miR-150-5p was compared to nc m; expression of cells transfected with mimic together with inhibitor was compared to nc m + i. ** p < 0.01, *** p < 0.001—unpaired t -test, r.u.—relative units. Expression of miR-150-5p and myb exerts a rhythmic patter with peak levels in antiphase in DLD1 cells. miR-150-5p mimic administration inhibits expression of bcl2 and myb .

Article Snippet: To evaluate the effect of miR-150-5p on apoptotic intensity, the human colorectal carcinoma cell line DLD1 (ATCC, Manassas, VA, USA) was used.

Techniques: Expressing, Transfection, Control, Sequencing

Journal: Biomedicines

Article Title: Daily Profile of miRNAs in the Rat Colon and In Silico Analysis of Their Possible Relationship to Colorectal Cancer

doi: 10.3390/biomedicines13081865

Figure Lengend Snippet: Effect of miR-150-5p mimic (150) and/or camptothecin (camp) on apoptosis and effect of miR-150-5p on wound healing and metabolism was tested in DLD1 cells. Representative images of cells with activated caspase 3 and/or 7 show effect of ( A ) control oligos (nc), ( B ) miR-150-5p mimic and camp together, and ( C ) camp alone. Images were taken with an inverted fluorescence microscope with a magnification of 100×. ( D ) summary of fluorescent staining showing apoptotic intensity after miR-150-5p mimic and/or camp administration. ( E ) effect of miR-150-5p on wound healing in DLD1 cell culture; ( F ) effect of miR-150-5p mimic, miR-150-5p inhibitor (150i), and miR-150-5p mimic together with inhibitor (150 + i) on metabolic activity of DLD1 cells. Histograms show mean ± SEM. Different letters above columns indicate significant differences between groups at the level of p < 0.05 calculated by ANOVA followed by Tukey’s post hoc test ( D ). Comparison between experimental oligos and corresponding negative control ( E , F ); * p < 0.05, ** p < 0.01—unpaired t -test. miR-150-5p facilitated early phase of camptothecin-induced apoptosis. Ectopic miR-150-5p decreased metabolic rate of DLD1 cells, and administration of inhibitor together with miR-150-5p mimic inverted this effect.

Article Snippet: To evaluate the effect of miR-150-5p on apoptotic intensity, the human colorectal carcinoma cell line DLD1 (ATCC, Manassas, VA, USA) was used.

Techniques: Control, Fluorescence, Microscopy, Staining, Cell Culture, Activity Assay, Comparison, Negative Control

Journal: Biomedicines

Article Title: Daily Profile of miRNAs in the Rat Colon and In Silico Analysis of Their Possible Relationship to Colorectal Cancer

doi: 10.3390/biomedicines13081865

Figure Lengend Snippet: Effect of miR-150-5p administration on expression of clock genes cry1 ( A ), cry2 ( B ), and per2 ( C ) in DLD1 cells. Cells were transfected with miR-150-5p (150) or miR-150-5p together with inhibitor (150 + i). Effect of miR-150-5p mimic or mimic administered together with inhibitor (oligo) was evaluated with respect to cells transfected with corresponding control sequences (nc). Comparison between experimental oligos and corresponding negative control; * p < 0.05—unpaired t -test, r.u.—relative units. miR-150-5p administration inhibited expression of cry1 and induced expression of cry2 and per2 .

Article Snippet: To evaluate the effect of miR-150-5p on apoptotic intensity, the human colorectal carcinoma cell line DLD1 (ATCC, Manassas, VA, USA) was used.

Techniques: Expressing, Transfection, Control, Comparison, Negative Control